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mpc11 murine mm cell line  (ATCC)


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    ATCC mpc11 murine mm cell line
    The cell lines <t>MPC11,</t> L363 and KMS12-BM were seeded at 10,000 cells/well in a 96-well plate. The cells were stimulated with control medium or Met(-)- medium. For the positive control (death), the cells were stimulated with 1 µM staurosporine. After 24 h, 48 h and 72 h, measurements were performed with the EarlyTox Live/Dead Assay Kit (Molecular Devices). The staining solution (5 µg/mL Hoechst 33342 (Thermo Fisher, Darmstadt, Germany), 6 µM EthD-III, and 6 µM calcein AM (CAM) in DMEM (Gibco, Life Technologies; Darmstadt, Germany)) was added, and after 30 min of incubation, measurements were made with the ImageXpress Pico Automated Cell Imaging System. The number of cells was determined via Hoechst 33342. Living cells were stained with the nonfluorescent calcein AM, which permeates the intact cell membrane and is converted into calcein, the fluorescent form, by intracellular esterases (cells are shown in green). If compromised cell membrane integrity is associated with cell death, EthD-III enters cells and binds to nucleic acids (cells are shown in red). A representative area for cell viability is shown for each cell line. The cell numbers are not representative of every case. The white bar, representing 52.44 µm, is used for size classification.
    Mpc11 Murine Mm Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mpc11+murine+mm+cell+line/MPC-11/bio_rxiv__2025__08__17__670755-54-1-10
    Average 96 stars, based on 258 article reviews
    mpc11 murine mm cell line - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Metabolic silencing via methionine-based amino acid restriction in multiple myeloma cell lines reveals a potential new strategy for cancer therapy"

    Article Title: Metabolic silencing via methionine-based amino acid restriction in multiple myeloma cell lines reveals a potential new strategy for cancer therapy

    Journal: bioRxiv

    doi: 10.1101/2025.08.17.670755

    The cell lines MPC11, L363 and KMS12-BM were seeded at 10,000 cells/well in a 96-well plate. The cells were stimulated with control medium or Met(-)- medium. For the positive control (death), the cells were stimulated with 1 µM staurosporine. After 24 h, 48 h and 72 h, measurements were performed with the EarlyTox Live/Dead Assay Kit (Molecular Devices). The staining solution (5 µg/mL Hoechst 33342 (Thermo Fisher, Darmstadt, Germany), 6 µM EthD-III, and 6 µM calcein AM (CAM) in DMEM (Gibco, Life Technologies; Darmstadt, Germany)) was added, and after 30 min of incubation, measurements were made with the ImageXpress Pico Automated Cell Imaging System. The number of cells was determined via Hoechst 33342. Living cells were stained with the nonfluorescent calcein AM, which permeates the intact cell membrane and is converted into calcein, the fluorescent form, by intracellular esterases (cells are shown in green). If compromised cell membrane integrity is associated with cell death, EthD-III enters cells and binds to nucleic acids (cells are shown in red). A representative area for cell viability is shown for each cell line. The cell numbers are not representative of every case. The white bar, representing 52.44 µm, is used for size classification.
    Figure Legend Snippet: The cell lines MPC11, L363 and KMS12-BM were seeded at 10,000 cells/well in a 96-well plate. The cells were stimulated with control medium or Met(-)- medium. For the positive control (death), the cells were stimulated with 1 µM staurosporine. After 24 h, 48 h and 72 h, measurements were performed with the EarlyTox Live/Dead Assay Kit (Molecular Devices). The staining solution (5 µg/mL Hoechst 33342 (Thermo Fisher, Darmstadt, Germany), 6 µM EthD-III, and 6 µM calcein AM (CAM) in DMEM (Gibco, Life Technologies; Darmstadt, Germany)) was added, and after 30 min of incubation, measurements were made with the ImageXpress Pico Automated Cell Imaging System. The number of cells was determined via Hoechst 33342. Living cells were stained with the nonfluorescent calcein AM, which permeates the intact cell membrane and is converted into calcein, the fluorescent form, by intracellular esterases (cells are shown in green). If compromised cell membrane integrity is associated with cell death, EthD-III enters cells and binds to nucleic acids (cells are shown in red). A representative area for cell viability is shown for each cell line. The cell numbers are not representative of every case. The white bar, representing 52.44 µm, is used for size classification.

    Techniques Used: Control, Positive Control, Live Dead Assay, Staining, Incubation, Imaging, Membrane

    Related Articles

    Control:

    Article Title: Metabolic silencing via methionine-based amino acid restriction in multiple myeloma cell lines reveals a potential new strategy for cancer therapy
    Article Snippet: The MPC11 murine MM cell line was purchased from the American Type Culture Collection (ATCC), and the KMS12-BM and L363 human MM cell lines were purchased from the Leibniz Institute, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The MPC11 cells were cultured in DMEM, and the KMS12-BM and L363 cells were cultured in RPMI 1640 medium (both from Gibco, Life Technologies; Darmstadt, Germany) supplemented with 10% foetal calf serum (FCS; Sigma–Aldrich, Darmstadt, Germany) and 1% penicillin/streptomycin (P/S; 100 U/mL penicillin and 100 μg/mL streptomycin; Thermo Fisher Scientific, Darmstadt, Germany) at 37 °C in a humidified atmosphere containing 5% CO 2 .The MPC11 cells were cultured in DMEM, and the KMS12-BM and L363 cells were cultured in RPMI 1640 medium (both from Gibco, Life Technologies; Darmstadt, Germany) supplemented with 10% foetal calf serum (FCS; Sigma–Aldrich, Darmstadt, Germany) and 1% penicillin/streptomycin (P/S; 100 U/mL penicillin..

    Positive Control:

    Article Title: Metabolic silencing via methionine-based amino acid restriction in multiple myeloma cell lines reveals a potential new strategy for cancer therapy
    Article Snippet: The MPC11 murine MM cell line was purchased from the American Type Culture Collection (ATCC), and the KMS12-BM and L363 human MM cell lines were purchased from the Leibniz Institute, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The MPC11 cells were cultured in DMEM, and the KMS12-BM and L363 cells were cultured in RPMI 1640 medium (both from Gibco, Life Technologies; Darmstadt, Germany) supplemented with 10% foetal calf serum (FCS; Sigma–Aldrich, Darmstadt, Germany) and 1% penicillin/streptomycin (P/S; 100 U/mL penicillin and 100 μg/mL streptomycin; Thermo Fisher Scientific, Darmstadt, Germany) at 37 °C in a humidified atmosphere containing 5% CO 2 .The MPC11 cells were cultured in DMEM, and the KMS12-BM and L363 cells were cultured in RPMI 1640 medium (both from Gibco, Life Technologies; Darmstadt, Germany) supplemented with 10% foetal calf serum (FCS; Sigma–Aldrich, Darmstadt, Germany) and 1% penicillin/streptomycin (P/S; 100 U/mL penicillin..

    Live Dead Assay:

    Article Title: Metabolic silencing via methionine-based amino acid restriction in multiple myeloma cell lines reveals a potential new strategy for cancer therapy
    Article Snippet: The MPC11 murine MM cell line was purchased from the American Type Culture Collection (ATCC), and the KMS12-BM and L363 human MM cell lines were purchased from the Leibniz Institute, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The MPC11 cells were cultured in DMEM, and the KMS12-BM and L363 cells were cultured in RPMI 1640 medium (both from Gibco, Life Technologies; Darmstadt, Germany) supplemented with 10% foetal calf serum (FCS; Sigma–Aldrich, Darmstadt, Germany) and 1% penicillin/streptomycin (P/S; 100 U/mL penicillin and 100 μg/mL streptomycin; Thermo Fisher Scientific, Darmstadt, Germany) at 37 °C in a humidified atmosphere containing 5% CO 2 .The MPC11 cells were cultured in DMEM, and the KMS12-BM and L363 cells were cultured in RPMI 1640 medium (both from Gibco, Life Technologies; Darmstadt, Germany) supplemented with 10% foetal calf serum (FCS; Sigma–Aldrich, Darmstadt, Germany) and 1% penicillin/streptomycin (P/S; 100 U/mL penicillin..

    Staining:

    Article Title: Metabolic silencing via methionine-based amino acid restriction in multiple myeloma cell lines reveals a potential new strategy for cancer therapy
    Article Snippet: The MPC11 murine MM cell line was purchased from the American Type Culture Collection (ATCC), and the KMS12-BM and L363 human MM cell lines were purchased from the Leibniz Institute, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The MPC11 cells were cultured in DMEM, and the KMS12-BM and L363 cells were cultured in RPMI 1640 medium (both from Gibco, Life Technologies; Darmstadt, Germany) supplemented with 10% foetal calf serum (FCS; Sigma–Aldrich, Darmstadt, Germany) and 1% penicillin/streptomycin (P/S; 100 U/mL penicillin and 100 μg/mL streptomycin; Thermo Fisher Scientific, Darmstadt, Germany) at 37 °C in a humidified atmosphere containing 5% CO 2 .The MPC11 cells were cultured in DMEM, and the KMS12-BM and L363 cells were cultured in RPMI 1640 medium (both from Gibco, Life Technologies; Darmstadt, Germany) supplemented with 10% foetal calf serum (FCS; Sigma–Aldrich, Darmstadt, Germany) and 1% penicillin/streptomycin (P/S; 100 U/mL penicillin..

    Incubation:

    Article Title: Metabolic silencing via methionine-based amino acid restriction in multiple myeloma cell lines reveals a potential new strategy for cancer therapy
    Article Snippet: The MPC11 murine MM cell line was purchased from the American Type Culture Collection (ATCC), and the KMS12-BM and L363 human MM cell lines were purchased from the Leibniz Institute, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The MPC11 cells were cultured in DMEM, and the KMS12-BM and L363 cells were cultured in RPMI 1640 medium (both from Gibco, Life Technologies; Darmstadt, Germany) supplemented with 10% foetal calf serum (FCS; Sigma–Aldrich, Darmstadt, Germany) and 1% penicillin/streptomycin (P/S; 100 U/mL penicillin and 100 μg/mL streptomycin; Thermo Fisher Scientific, Darmstadt, Germany) at 37 °C in a humidified atmosphere containing 5% CO 2 .The MPC11 cells were cultured in DMEM, and the KMS12-BM and L363 cells were cultured in RPMI 1640 medium (both from Gibco, Life Technologies; Darmstadt, Germany) supplemented with 10% foetal calf serum (FCS; Sigma–Aldrich, Darmstadt, Germany) and 1% penicillin/streptomycin (P/S; 100 U/mL penicillin..

    Imaging:

    Article Title: Metabolic silencing via methionine-based amino acid restriction in multiple myeloma cell lines reveals a potential new strategy for cancer therapy
    Article Snippet: The MPC11 murine MM cell line was purchased from the American Type Culture Collection (ATCC), and the KMS12-BM and L363 human MM cell lines were purchased from the Leibniz Institute, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The MPC11 cells were cultured in DMEM, and the KMS12-BM and L363 cells were cultured in RPMI 1640 medium (both from Gibco, Life Technologies; Darmstadt, Germany) supplemented with 10% foetal calf serum (FCS; Sigma–Aldrich, Darmstadt, Germany) and 1% penicillin/streptomycin (P/S; 100 U/mL penicillin and 100 μg/mL streptomycin; Thermo Fisher Scientific, Darmstadt, Germany) at 37 °C in a humidified atmosphere containing 5% CO 2 .The MPC11 cells were cultured in DMEM, and the KMS12-BM and L363 cells were cultured in RPMI 1640 medium (both from Gibco, Life Technologies; Darmstadt, Germany) supplemented with 10% foetal calf serum (FCS; Sigma–Aldrich, Darmstadt, Germany) and 1% penicillin/streptomycin (P/S; 100 U/mL penicillin..

    Membrane:

    Article Title: Metabolic silencing via methionine-based amino acid restriction in multiple myeloma cell lines reveals a potential new strategy for cancer therapy
    Article Snippet: The MPC11 murine MM cell line was purchased from the American Type Culture Collection (ATCC), and the KMS12-BM and L363 human MM cell lines were purchased from the Leibniz Institute, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The MPC11 cells were cultured in DMEM, and the KMS12-BM and L363 cells were cultured in RPMI 1640 medium (both from Gibco, Life Technologies; Darmstadt, Germany) supplemented with 10% foetal calf serum (FCS; Sigma–Aldrich, Darmstadt, Germany) and 1% penicillin/streptomycin (P/S; 100 U/mL penicillin and 100 μg/mL streptomycin; Thermo Fisher Scientific, Darmstadt, Germany) at 37 °C in a humidified atmosphere containing 5% CO 2 .The MPC11 cells were cultured in DMEM, and the KMS12-BM and L363 cells were cultured in RPMI 1640 medium (both from Gibco, Life Technologies; Darmstadt, Germany) supplemented with 10% foetal calf serum (FCS; Sigma–Aldrich, Darmstadt, Germany) and 1% penicillin/streptomycin (P/S; 100 U/mL penicillin..



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    The cell lines <t>MPC11,</t> L363 and KMS12-BM were seeded at 10,000 cells/well in a 96-well plate. The cells were stimulated with control medium or Met(-)- medium. For the positive control (death), the cells were stimulated with 1 µM staurosporine. After 24 h, 48 h and 72 h, measurements were performed with the EarlyTox Live/Dead Assay Kit (Molecular Devices). The staining solution (5 µg/mL Hoechst 33342 (Thermo Fisher, Darmstadt, Germany), 6 µM EthD-III, and 6 µM calcein AM (CAM) in DMEM (Gibco, Life Technologies; Darmstadt, Germany)) was added, and after 30 min of incubation, measurements were made with the ImageXpress Pico Automated Cell Imaging System. The number of cells was determined via Hoechst 33342. Living cells were stained with the nonfluorescent calcein AM, which permeates the intact cell membrane and is converted into calcein, the fluorescent form, by intracellular esterases (cells are shown in green). If compromised cell membrane integrity is associated with cell death, EthD-III enters cells and binds to nucleic acids (cells are shown in red). A representative area for cell viability is shown for each cell line. The cell numbers are not representative of every case. The white bar, representing 52.44 µm, is used for size classification.
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    Image Search Results


    The cell lines MPC11, L363 and KMS12-BM were seeded at 10,000 cells/well in a 96-well plate. The cells were stimulated with control medium or Met(-)- medium. For the positive control (death), the cells were stimulated with 1 µM staurosporine. After 24 h, 48 h and 72 h, measurements were performed with the EarlyTox Live/Dead Assay Kit (Molecular Devices). The staining solution (5 µg/mL Hoechst 33342 (Thermo Fisher, Darmstadt, Germany), 6 µM EthD-III, and 6 µM calcein AM (CAM) in DMEM (Gibco, Life Technologies; Darmstadt, Germany)) was added, and after 30 min of incubation, measurements were made with the ImageXpress Pico Automated Cell Imaging System. The number of cells was determined via Hoechst 33342. Living cells were stained with the nonfluorescent calcein AM, which permeates the intact cell membrane and is converted into calcein, the fluorescent form, by intracellular esterases (cells are shown in green). If compromised cell membrane integrity is associated with cell death, EthD-III enters cells and binds to nucleic acids (cells are shown in red). A representative area for cell viability is shown for each cell line. The cell numbers are not representative of every case. The white bar, representing 52.44 µm, is used for size classification.

    Journal: bioRxiv

    Article Title: Metabolic silencing via methionine-based amino acid restriction in multiple myeloma cell lines reveals a potential new strategy for cancer therapy

    doi: 10.1101/2025.08.17.670755

    Figure Lengend Snippet: The cell lines MPC11, L363 and KMS12-BM were seeded at 10,000 cells/well in a 96-well plate. The cells were stimulated with control medium or Met(-)- medium. For the positive control (death), the cells were stimulated with 1 µM staurosporine. After 24 h, 48 h and 72 h, measurements were performed with the EarlyTox Live/Dead Assay Kit (Molecular Devices). The staining solution (5 µg/mL Hoechst 33342 (Thermo Fisher, Darmstadt, Germany), 6 µM EthD-III, and 6 µM calcein AM (CAM) in DMEM (Gibco, Life Technologies; Darmstadt, Germany)) was added, and after 30 min of incubation, measurements were made with the ImageXpress Pico Automated Cell Imaging System. The number of cells was determined via Hoechst 33342. Living cells were stained with the nonfluorescent calcein AM, which permeates the intact cell membrane and is converted into calcein, the fluorescent form, by intracellular esterases (cells are shown in green). If compromised cell membrane integrity is associated with cell death, EthD-III enters cells and binds to nucleic acids (cells are shown in red). A representative area for cell viability is shown for each cell line. The cell numbers are not representative of every case. The white bar, representing 52.44 µm, is used for size classification.

    Article Snippet: The MPC11 murine MM cell line was purchased from the American Type Culture Collection (ATCC), and the KMS12-BM and L363 human MM cell lines were purchased from the Leibniz Institute, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).

    Techniques: Control, Positive Control, Live Dead Assay, Staining, Incubation, Imaging, Membrane

    The biological activity of BMP2 was evaluated in two well-established cell systems. (A) BMP2-induced ALP expression in murine C2C12 cells. (B) The anti-proliferative effect induced by BMP2 in murine MPC11 cells was determined from DNA synthesis as measured via 3H-thymidine incorporation.

    Journal: PLoS ONE

    Article Title: The anti-myeloma activity of bone morphogenetic protein 2 predominantly relies on the induction of growth arrest and is apoptosis-independent

    doi: 10.1371/journal.pone.0185720

    Figure Lengend Snippet: The biological activity of BMP2 was evaluated in two well-established cell systems. (A) BMP2-induced ALP expression in murine C2C12 cells. (B) The anti-proliferative effect induced by BMP2 in murine MPC11 cells was determined from DNA synthesis as measured via 3H-thymidine incorporation.

    Article Snippet: The murine MM cell line MPC11 and Jurkat A3 cells were obtained from the ATCC collection (LGC Standards GmbH, Germany).

    Techniques: Activity Assay, Expressing, DNA Synthesis